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Literature summary extracted from

  • Shen, Y.; Han, Y.J.; Kim, J.I.; Song, P.S.
    Arabidopsis nucleoside diphosphate kinase-2 as a plant GTPase activating protein (2008), BMB Rep., 41, 645-650.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.6.5.2 nucleoside diphosphate kinase-2 EC 2.7.4.6 Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.6 expression of GST-tagged NPDK2 Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
2.7.4.6 H197C NDPK2 kinase site mutant Arabidopsis thaliana
2.7.4.6 H197C no enzymatic activity, stimulates GTPase activites of Pra2 and Pra3, EC 3.6.5.2, in the same manner as wild-type NDPK2 Arabidopsis thaliana
2.7.4.6 L225stop C-terminal deletion mutant Arabidopsis thaliana
2.7.4.6 L225stop increased enzymatic activity, stimulates GTPase activites of Pra2 and Pra3, EC 3.6.5.2, in the same manner as wild-type NDPK2 Arabidopsis thaliana
2.7.4.6 LP175S no interaction between NDPK2 and small G proteins, EC 3.6.5.2 Arabidopsis thaliana
2.7.4.6 P175S NDPK2 kinase site mutant Arabidopsis thaliana
2.7.4.6 P175S stimulates GTPase activites of Pra2 and Pra3, EC 3.6.5.2, with a slight reduction compared to wild-type NDPK2 Arabidopsis thaliana
2.7.4.6 R230stop C-terminal deletion mutant Arabidopsis thaliana
2.7.4.6 R230stop increased enzymatic activity, stimulates GTPase activites of Pra2 and Pra3, EC 3.6.5.2, in the same manner as wild-type NDPK2 Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.6 Mg2+
-
 Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.6 additional information Arabidopsis thaliana NDPK is involved in multiple signaling pathways in mammalian systems, including G-protein signaling. Physical interaction between NDPK2 and small G proteins, as well as the heterotrimeric G protein GPA1. NDPK2 can stimulate their GTPase activities, but the dimeric form of NDPK2 is also unable to enhance the GTPase activities of both Pra2 and Pra3, the role oligomeric states may play in the interaction of NDPK2 with small G proteins ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.6 Arabidopsis thaliana
-
-
-
2.7.4.6 Arabidopsis thaliana O64903
-
-
3.6.5.1 Arabidopsis thaliana
-
-
-
3.6.5.2 Arabidopsis thaliana
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.6 additional information NDPK is involved in multiple signaling pathways in mammalian systems, including G-protein signaling. Physical interaction between NDPK2 and small G proteins, as well as the heterotrimeric G protein GPA1. NDPK2 can stimulate their GTPase activities, but the dimeric form of NDPK2 is also unable to enhance the GTPase activities of both Pra2 and Pra3, the role oligomeric states may play in the interaction of NDPK2 with small G proteins Arabidopsis thaliana ?
-
 ?
2.7.4.6 additional information NDPK2 interacts with plant small G proteins in vitro, both Pra2 and Pra3both Pra2 and Pra3, the role oligomeric states may play in the interaction of NDPK2 with small G proteins Arabidopsis thaliana ?
-
 ?
3.6.5.1 GTP + H2O assay at pH 7.8, 30°C, 30 min Arabidopsis thaliana GDP + phosphate
-
 ?
3.6.5.2 GTP + H2O assay at pH 7.8, 30°C, 30 min Arabidopsis thaliana GDP + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.4.6 hexamer
-
 Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
2.7.4.6 NDPK2
-
 Arabidopsis thaliana
2.7.4.6 nucleoside diphosphate kinase
-
 Arabidopsis thaliana
2.7.4.6 nucleoside diphosphate kinase-2
-
 Arabidopsis thaliana
3.6.5.1 GPA1
-
 Arabidopsis thaliana
3.6.5.1 GTPase
-
 Arabidopsis thaliana
3.6.5.1 heterotrimeric G protein alpha subunit
-
 Arabidopsis thaliana
3.6.5.2 GTPase
-
 Arabidopsis thaliana
3.6.5.2 Pra2
-
 Arabidopsis thaliana
3.6.5.2 Pra3
-
 Arabidopsis thaliana
3.6.5.2 small G protein
-
 Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.6 7.5
-
 assay at Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.4.6 ATP
-
 Arabidopsis thaliana